Abstract
immutans (im) is a recessive Arabidopsis mutant that is involved in chloroplast biogenesis. In the absence of IMMUTANS (IM) green and white leaf sectors form. This thesis is concerned with the development of these green and white sectors and the methods used to investigate the mechanism of their formation. IM resides in the chloroplast thylakoid membrane and has been implicated in the adjustment of electron flux through the photosynthetic electron transport chain. Without IM white sector formation occurs due to the inability of im to produce colored carotenoids. It is still a mystery as to how green sectors are able to form in the absence of IM. This thesis presents im as a developmental chloroplast defect and discusses the need for IM very early in plastid development. The requirement for IM most likely starts within progenitor plastids known as proplastids.
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