Abstract
The α-amino acid nitrogen to creatinine nitrogen ratio of normal rats on an ad libitum diet is quite constant and provides a reliable method for determining alterations in amino acid excretion. Exposure of rats to high levels of CCl 4 vapor results in increased urinary excretion of free amino acids. There is a simultaneous increase in the corresponding free amino acids of the blood suggesting that increased urinary excretion is a reflection of elevated levels of free amino acids in the blood. The suggestion is made that the source of the elevated serum free amino acids is damaged tissue and, on the basis of the hepatotoxic action of CCl 4, one would expect damaged liver cells to be the major source of these free amino acids.
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