A study of bionics micro-textures on the surface of HA bio-coatings prepared by nanosecond laser

Abstract

The material and surface morphology of bone implants can influence cellular activity and proliferation properties. The preparation of biomimetic microtextures on the hydroxyapatite (HA) bio-coated surface can enhance the biological properties of the implant. In this study, a nanosecond laser was used to prepare microtextures on the surface of HA coatings, and the effect of laser parameters on the ablation morphology was investigated by micromorphological observation and response surface methods. The ablation morphology was found to be better when the laser repetition frequency was 20 kHZ, the scanning speed was 300 mm/s and the number of scans was 7. Microtextures imitating fish scales and crocodile skin of different sizes were prepared on the surface of HA coatings using this laser parameter. The biological properties of the microtextures were investigated using CCK-8 tests and cell adhesion tests and the nanosecond laser-prepared mimetic microtextures were found to promote cell proliferation. A 0.1 mm radius of the mock fish scale microtexture had optimal cellular activity, where the tentacles of the cells could fully extend to the surface of the microstructure, which facilitated cell adhesion and proliferation.