Abstract
This study was carried out at the college of agriculture and marine science centre, Basrah university. The present study was conducted to investigate the existence of polymorphism at transferrin (Tf) locus in the Carp (Cyprinus carpio). A polyacrylamide gel electrophoresis (PAGE) under alkaline condition method was used to distinguish Carp Tf alleles. Use the gel documentation program in this study. Analysis of 116 animals revealed that all animals were polymorphic, showing many genotypes. There was very clear biodiversity in the Tf gene. Seven Tf genotypes consisting of 4 homozygote types (CC, DD, FF and GG) and two heterozygote types (CD, DG and FG) were detected. These fractions are controlled by co-dominant autosomal genes according to the Mendelian laws of inheritance. The highest gene frequencies were calculated 0.50 for Tf D, 0.26 for Tf F and 0.12 for C and G. thus, carp (Cyprinus carpio) assemblages consistently tended to be more predominant to D allele. Differences between expected number and observed number for transferrin genotypes were no significant. This is useful in genetic improvement process through the selection. As far as we know, this is the first large-scale analysis on the genetic polymorphism in carp (Cyprinus carpio). Polyacrylamide electrophoresis, the technique employed in this study, allows rapid and efficient screening for the presence of polymorphism in Tf.
Highlights
Transferrin, one of class I genetic markers, is the most heterogeneous polymorphic blood protein in Carp (Valenta et al, 1976 and Csizmadia et al, 1995), in goose (Valenta and Stratil, 1978), in chicken (Vyshinsky and Muravjev, 1970) and sheep (Jaayid et al, 2011), a total of 7 co-dominant alleles have been found in its locus
The Carp (Cyprinus carpio) transferrin types were named according to the nomenclature suggested by Irnazarow and Bialowas (1994) and Jurecka et al (2009)
Gene frequencies were calculated by the method of gene counting as the mode of inheritance of each of the systems that do show variation is that of codominant alleles at an autosomal locus (Khaertdinov, 2000)
Summary
Transferrin, one of class I genetic markers, is the most heterogeneous polymorphic blood protein in Carp (Valenta et al, 1976 and Csizmadia et al, 1995), in goose (Valenta and Stratil, 1978), in chicken (Vyshinsky and Muravjev, 1970) and sheep (Jaayid et al, 2011), a total of 7 co-dominant alleles have been found in its locus. Transferrin polymorphism was demonstrated in different breeds in fish. The aim of these survey was to describe the polymorphism of transferrins of carp strains in the live gene bank.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
