Abstract
Aims: To investigate the changes in the protein composition of aqueous humour in primary open angle glaucoma patients and non-glaucomatous subjects. Patients and Methods: Case control study was conducted at a university hospital to compare the protein profile of aqueous humour obtained from patients of primary open angle glaucoma with those of non glaucomatous subjects. Protein concentration of the aqueous humour samples collected from both glaucomatous and non-glaucomatous patients at the time of surgery was estimated by the Bradford protein assay method. Sodium dodecylsulfate polyacrylamide gel electrophoresis of the samples was also performed. Results: Geometric mean of protein concentration of the samples was 55.73 mg/dl (range: 31-72) in the test group and 46.46 mg/dl (range: 27-65) in the control group and a statistically significant (p ≤= 0.001) difference in protein concentration of aqueous humour between the two groups was observed. Electrophoretic study revealed differences in the aqueous protein composition of the two groups. The glaucomatous group exhibited protein bands of 10 kDa, 20 kDa, 30 kDa, 50 kDa, 60 kDa, 70 kDa, and 90 kDa while the control subjects demonstrated bands of 6 kDa, 10 kDa, 30 kDa, 70 kDa, 90 kDa. Interestingly, almost all the proteins detected in this study corresponded to the molecular weights of heat shock proteins. Conclusions: Primary open angle glaucoma patients differed in protein compositions and had higher concentration of aqueous humour proteins than non glaucomatous patients.
Highlights
Adult-onset primary open-angle glaucoma (POAG) is the most common form of glaucoma, usually manifesting itself after the age of 40 years
Due to lack of sufficient information on the role of proteins involved in the pathogenesis of open angle glaucoma, the present study was undertaken to assess the changes in the protein composition of aqueous humour of primary open angle glaucoma and to compare it with those of non-glaucomatous patients
Outflow facility is reported to be significantly reduced in patients with uveitis who have high aquous humour protein level
Summary
Adult-onset primary open-angle glaucoma (POAG) is the most common form of glaucoma, usually manifesting itself after the age of 40 years. Compared to other forms of glaucoma, the optic neuropathy of POAG is multifactorial, with IOP, being the most important risk factor [3]. It is generally believed that like all other forms of glaucoma, it is a mechanical blockade of aqueous outflow that increase IOP of POAG. The total protein concentration in primary open-angle glaucoma aqueous humour was approximately two times higher than that in non-glaucomatous subjects [4]. Due to lack of sufficient information on the role of proteins involved in the pathogenesis of open angle glaucoma (especially POAG), the present study was undertaken to assess the changes in the protein composition of aqueous humour of primary open angle glaucoma and to compare it with those of non-glaucomatous patients. A better understanding of the mechanism of POAG is likely to lead to more effective treatment of this disease
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