A Study Of antioxidant capacity of different doses of alcoholic extract of Marticaria Chamomilla flower in comparison with vitamin E in oxidative stressed male rats

Abstract

This study was conducted to investigate the effect of alcoholic extract of Marticaria chamomilla flowers on the antioxidant status in comparison with vitamin E in male rats. Thirty mature male Albino Wister rats were used in this study, the animals were divided into six groups (5 each), the first group was administered with water only, the second one was subjected to H2O2 0.75% in drinking water. The third group was orally intubated with 50mg/kg vitamin E concurrently with H2O2. The other three groups (T3, T4 and T5) were orally administered with M.Ch.E (40, 80 and 120 mg/kg BW) respectively. Blood samples were taken at zero time and then after 30 days, serum sample were examined for CAT, peroxynitrite radical concentration, TC, TAG, HDL-C, VLDL-C and LDL-C. The results showed a significant elevation in ONOO concentration, TC, TAG, VLDL-C and LDL-C associated with a reduction in CAT and HDL-C in H2O2 treated group in compared with control group, this result may be contributed to oxidative damage associated with long term H2O2 exposure. While other groups (vitamin E group and M.Ch.E treated groups) showed a significant reduction in ONOO concentration, TC, TAG, VLDL-C and LDL-C with an elevation in HDL-C if compared to H2O2 treaded group. This may be contributed to the antioxidant effect of vitamin E and M.Ch.E. However, 120mg/kg BW M.Ch.E treated group recorded the highest positive effect against oxidative stress and its effect seems to be normalizing with vitamin E effect, as well as both groups (vit. E and 120mg/kg BW M.Ch.E) clarified a non-significant different with control group in all experiment parameters. This result pointed to antioxidant effect of M.Ch.E in a dose dependent manner.