Abstract
The photochemical stability of Gomori Aldehyde Fuschin stain samples of different ages was investigated by thin layer, column and high performance liquid chromatographic techniques and the component dyes isolated. The dyes obtained were individually and sequentially irradiated with simulated sunlight and the photodegradation products isolated and characterised by comparison of infra red spectra with those of authentic samples. Pararosaniline was found to undergo progressive methylation leading eventually to the formation of Crystal Violet which was subsequently ethylated to Methyl Green. Methyl Green was found to undergo disproportionation to Malachite Green. The histochemical consequences of these reactions are discussed.
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