Abstract
Two different molecular forms of adenosine deaminase have been found in human tissues: the high molecular weight enzyme and the low molecular weight enzyme, designated as the A form and the C form respectively. These two enzyme forms are present in various proportions in different tissues. In previous studies, the two enzyme forms were separated and identified by gel filtration column chromatography and spectrophotometric measurements of enzyme activity in the elution fractions. A rapid and efficient method is needed to perform routine analysis of the enzyme form distribution, especially in samples containing little enzyme activity, and to study the interrelationship of the two enzyme forms. This study shows the development of the thin-layer gel filtration technique for the separation and the identification of the two molecular forms of adenosine deaminase in human tissue extracts. Possible clinical application of this technique and the enzyme system is discussed. This work was supported by a grant from the Indiana Delaware County Cancer Society.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
