Abstract
Plasma from three patients with hemophilia B and acquired factor IX inhibitors interfered with the reaction between rabbit antiserum against factor IX and factor IX in immunoelectrophoretic techniques. The inhibitors did not precipitate factor IX in diffusion-in-gel experiments, but behaved like IgG on DEAE ion chromatography. Incorporation of inhibitor plasma in the agarose gel in the first dimension in crossed immunoelectrophoresis reduced the electrophoretic mobility of factor IX antigen. The reduction of the mobility was dependent on the concentration of inhibitor plasma in the agarose. Incorporation of inhibitor plasma in an intermediate gel in “rocket” immunoelectrophoresis gave a concentration dependent reduction of the size of the “rockets”. Both techniques could be used for determination of a titre of the inhibitors which was in agreement with the titre determined in a coagulation assay.
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