Abstract
A Study in the Viability of Butea monosperma Seeds
Highlights
A Study in the Viability of Butea Monosperma Seeds1*Department of Botany , Shri Umiya Kanya Mahavidalay, Indore, India 2Department of Botany, Dr Hari Singh Gour University, Sagar, India
Seed viability is usually assessed by means of a standard germination test requiring a minimum of 3 weeks
Viability has to be determined by some alternative and more reliable methods, which may be less time consuming as well as can be applied to large seed samples
Summary
1*Department of Botany , Shri Umiya Kanya Mahavidalay, Indore, India 2Department of Botany, Dr Hari Singh Gour University, Sagar, India. The. Tetrazolium Test involves the use of [2, 3, 5], triphenyl tetrazolium chloride which in a living cell is reduced by the dehydrogenase enzyme system. Tetrazolium Test involves the use of [2, 3, 5], triphenyl tetrazolium chloride which in a living cell is reduced by the dehydrogenase enzyme system This reaction yields a red compound called triphenyl formazan. Since this reaction is enzyme mediated the cell in which it is taking place has to be a liveing cell. Stained areas of a tissue in this test indicate occurrence of live cells and unstained ones the necrotic tissue.
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