A study about the relationship between bFGF and IL-6/STAT3 signaling pathway in glioma cells in vitro

Abstract

Objective To investigate the role of the relationship between basic fibroblast growth factor and IL-6/STAT3 signaling pathway in glioma cells.Methods U251 cells were divided into the control group,mock group and experimental group.The mock and experimental groups were transfected with mock vector(Ad-GFP) and the recombinant adenovirus carrying bFGF-siRNA (Ad-bFGF-siRNA) respectively at a multiplicity of infection (MOI) of 100.The expression of related proteins was revealed by the method of Western blot,and the levels of IL-6 in the supernatant of U251 cells were measured via the method of ELISA.Results After U251 cells were transfected with bFGF-siRNA,the results of Western blot showed that compared with the control group and empty vector group pSTAT3 (Tyr705) and pSTAT3 (Ser727) expression decreased,which was time dependent,also the expression of STAT3 upstream kinase pERK1/2,pJAK2,Src and the downstream effector proteins CyclinD1,Bcl-xl were decreased; the IL-6 level in U251 cell supernatant detection via ELISA showed in the experimental group IL-6 concentration decreased obviously,the differences were statistically significant (P ＜ 0.05).Conclusions Adenovirus mediated small interfering RNA targeting bFGF could reduce the activation of STAT3 signaling pathway in glioma U251 cell line by suppression of IL-6 secretion,as well as inhibit the expression of the upstream regulating kinase JAK2,ERK and downstream effector molecules CyclinD1 and Bcl-xl. Key words: Basic fibroblast growth factor; Interleukin-6; Signal transducer and activators of transcription-3 ; Glioma