Abstract
TSH receptors from guinea pig thyroid and epididymal fat have been covalently crosslinked to 125I-labelled TSH conjugated to N-hydroxysuccinimidyl-4-azidobenzoate. Analysis by SDS—PAGE and autoradiography showed bands corresponding to TSH subunits ( M r 14 000) and intact TSH ( M r 28 000; subunits crosslinked) and two at higher M r separated by 14 000. The latter bands represented one or two subunits of TSH crosslinked to a subunit of the TSH receptor with M r 57 000 (fat) or 60 000 (thyroid). These M r values were reduced by trypsin treatment to 43 000 and 50 000, respectively. Analysis under non-reducing conditions showed that both fat and thyroid receptors have a second disulphide linked subunit of M r 30 000.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
