Abstract
Ten compounds, including three lignan glycosides and seven lignans, were purified from Justicia procumbens L. in 8 h using an efficient strategy based on high-speed counter-current chromatography (HSCCC). The two-phase solvent system composed of petroleum–ethyl acetate–methanol–H2O (1:0.7:1:0.7, v/v) was firstly employed to separate the crude extract (320 mg), from which 19.3 mg of justicidin B (f), 10.8 mg of justicidin A (g), 13.9 mg of 6′-hydroxyjusticidin C (h), 7.7 mg of justicidin E (i), 6.3 mg of lignan J1 (j) were obtained with 91.3 mg of enriched mixture of compounds a–e. The enriched mixture (91.3 mg) was further separated using the solvent system consisting of petroleum–ethyl acetate–methanol–H2O (3:3.8:3:3.8, v/v), yielding 12.1 mg of procumbenoside E (a); 7.6 mg of diphyllin-1-O-β-d-apiofuranoside (b); 7.4 mg of diphyllin (c); 8.3 mg of 6′-hydroxy justicidin B (d); and 7.9 mg of diphyllin acetyl apioside (e). The purities of the 10 components were all above 94%, and their structures were identified by NMR and ESI-MS spectra. The results demonstrated that the strategy based on HSCCC for the separation of lignans and their glycosides was efficient and rapid.
Highlights
Justicia procumbens L. (Chinese name Juechuang) is one of the famous traditional Chinese herbal medicine, which stemmed from the dried whole plant of the genus Acanthaceae [1]
Selectionof ofsolvent solventsystem systemisisthe thekey keystep stepfor forthe theHSCCC
The study is of great reference value for obtaining high purity lignans and their glycosides from J. procumbens, which indicated that high-speed counter-current chromatography (HSCCC) is a powerful technique for the separation and purification of active compounds with a wide range of polarity from natural products
Summary
Justicia procumbens L. (Chinese name Juechuang) is one of the famous traditional Chinese herbal medicine, which stemmed from the dried whole plant of the genus Acanthaceae [1]. (Chinese name Juechuang) is one of the famous traditional Chinese herbal medicine, which stemmed from the dried whole plant of the genus Acanthaceae [1]. It has detoxification and diuretic swelling effect for the treatment of fever, cough, sore throat, cirrhosis of the ascites [2,3]. Previous phytochemical studies indicated that J. procumbens contains various ingredients, including lignans, lignan glycosides, alkaloids, flavonoids, triterpenes, steroids, etc. Modern pharmacological investigations indicated that lignans and their glycosides are the main chemical components in J. procumbens, which exhibit various pharmacological activities, including antitumor, antivirus, antihepatitis, inhibition of platelet aggregation, and cytotoxicity [7,8,9,10]. In order to better understand the biochemical properties of lignans and their glycosides from J. procumbens and to ascertain their clinical applications, it is essential to develop an efficient method for the preparative separation of lignans and their glycosides.
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