Abstract
The availability of engineered biological tissues holds great potential for both clinical applications and basic research in a life science laboratory. A prototype standalone perfusion/compression bioreactor system was proposed to address the osteogenic commitment of stem cells seeded onboard of 3D chitosan-graphene (CHT/G) templates. Testing involved the coordinated administration of a 1 mL/min medium flow rate together with dynamic compression (1% strain at 1 Hz; applied twice daily for 30 min) for one week. When compared to traditional static culture conditions, the application of perfusion and compression stimuli to human bone marrow stem cells using the 3D CHT/G template scaffold induced a sizable effect. After using the dynamic culture protocol, there was evidence of a larger number of viable cells within the inner core of the scaffold and of enhanced extracellular matrix mineralization. These observations show that our novel device would be suitable for addressing and investigating the osteogenic phenotype commitment of stem cells, for both potential clinical applications and basic research.
Highlights
Sciences and Technologies - Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Via Tolara di Sopra 41/E, 40064, Ozzano dell’Emilia, BO, Italy. 6The Blood Bank, The Landspitali University Hospital, Snorrabraut 60, 105, Reykjavík, Iceland. 7Present address: Center of Medical Physics and Biomedical Engineering, Medical University of Vienna, Waehringer Guertel 18-20/4L, 1090, Wien, Austria. 8These authors contributed : The bioreactor system described uses 3D-printed custom chambers to host the tissue constructs
Building on our experience[8,18,19,20,21,22,41] we have developed a standalone perfusion and compression bioreactor system to enhance the cell viability and extracellular matrix (ECM) mineralization of mesenchymal stem cells (MSCs) cultured within 3D chitosan graphene (CHT/G) scaffold templates
The bioreactor system detailed in this manuscript is a novel standalone device that allows culturing of 3D cell constructs within a controlled environment, where tightly regulated medium perfusion and scaffold compression are intended to sustain human bone marrow-derived mesenchymal stem cell survival, proliferation, and commitment towards an osteogenic phenotype
Summary
Sciences and Technologies - Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Via Tolara di Sopra 41/E, 40064, Ozzano dell’Emilia, BO, Italy. 6The Blood Bank, The Landspitali University Hospital, Snorrabraut 60, 105, Reykjavík, Iceland. 7Present address: Center of Medical Physics and Biomedical Engineering, Medical University of Vienna, Waehringer Guertel 18-20/4L, 1090, Wien, Austria. 8These authors contributed : The bioreactor system described uses 3D-printed custom chambers to host the tissue constructs. Sciences and Technologies - Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Via Tolara di Sopra 41/E, 40064, Ozzano dell’Emilia, BO, Italy. These allow the administration of perfusion flow through a dedicated peristaltic pump and application of a compressive axial deformation through a custom-made mechanical loading platform driven by a precise stepper motor. Enhanced cell viability and initial ECM mineralization was evident as early as 1 week into treatment, indicating that this prototype may be suitable to implement as a standard method for rapidly inducing and/or investigating osteogenic phenotype commitment
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
