Abstract
A simple, sensitive, and stability-indicating thin-layer chromatographic (TLC)-densitometric method was developed for the assay of biomarker bergenin in the methanol extract of aerial parts of Flueggea virosa (FVME). Chromatography was performed on glass-backed silica gel 60 F254 high-performance thin-layer chromatographic (HPTLC) plates with dichloromethane-methanol (8.5:1.5, v/v) as the mobile phase. Scanning and quantification were done at 220 nm. The system was found to give compact spot for bergenin at RF = 0.29 ± 0.01. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.998 with respect to area in the concentration range of 100–800 ng. The regression equation of bergenin was found to be Y = 8.708X + 51.017. The limit of detection (LOD) and limit of quantification (LOQ) for bergenin were found to be 17.5 and 53 ng band−1, respectively. The percentage of bergenin was found to be 15.5% w/w in the FVME. Bergenin was subjected to acid and alkali hydro...
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