Abstract
Embryonic aphids are a good source of aphid chromosomes when extruded and left for 15 minutes in 25% Ringer's solution, fixed for 1 hour in a mixture of 2 parts glacial acetic acid, 2 parts 95% ethyl alcohol, and 0.125 parts of chloroform. The embryos are then hydrolyzed for 5 minutes in normal hydrochloric acid at 60 °C. and stained in Gomori's hematoxylin for 30 minutes at 60 °C. After differentiation in 45% acetic acid for [Formula: see text]to 2 hours, the embryos are placed on a microscope slide, squashed under a cover glass, and mounted in 45% acetic acid. Individual plates are spread and flattened at low magnification with the point of a scalpel by tapping and pressing the cover glass over the figure. Unused embryos in 45% acetic acid and prepared slides will keep in "deep freeze" for about two weeks. Permanent preparations are made by floating the cover glasses from frozen slides in cold 95% ethyl alcohol, dehydrating in absolute alcohol, and mounting in an alcohol-based mounting medium.
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