A SPR Glucose Sensor Based on Immobilized Glucose Oxidases and Silica Mesocellular Foams

Abstract

An improved glucose-sensitive membrane (GSM) was prepared by immobilizing glucose oxidases (GODs) onto silica mesocellular foams and trapping them in a polyacrylamide gel. This gel was then coated on a gold/glass sheet to realize surface plasmon resonance (SPR) sensors. A series of sensing experiments was conducted to obtain the optimized parameters with the improved GSM. The experimental results showed that the improved SPR glucose sensor has a sensitivity of 0.0135 degree/(mg/dL) and a linear range of 0–160 mg/dL. This linear range is twice that obtained with the GSM by immobilizing GODs on SiO2 nanoparticles.