A Spontaneous, Light Independent and Prolific Plant Regeneration Response from Hairy Roots of Nicotiana hesperis Transformed by Agrobacterium rhizogenes

Abstract

Summary Transformation of Nicotiana hesperis was induced by Agrobacterium rhizogenes . Hairy root cultures underwent prolific and spontaneous shoot regeneration in a medium lacking hormones both in light and in darkness. Excised seedling roots of N. hesperis also exhibited spontaneous shoot regeneration. Plants derived from hairy root tissue contained Ri T-DNA and showed a typical altered morphology with wrinkled leaves, reduced height at flowering and reduced seed set.