Abstract
The diabetic wounds do not heal easily in part because they are susceptible to infection due to environmental influences. Wound dressing is crucial to wound healing, as it can basically protect the wound from external damages and provide a suitable microenvironment for tissue regeneration. In this study, a double-layer membrane that consists of chitosan sponge and decellularized bovine amniotic membrane (dBAM) has been developed by freeze-casting method. The results showed that the porous structure of the sponge layer improved the performances of blood coagulation and swelling. The dense dBAM can optimize the mechanical property of wound dressing. In vitro studies revealed that the bilayer membrane had favorable biocompatible, especially for human foreskin fibroblast cells (HFF-1) cell adhesion and proliferation. Moreover, the full-thickness skin defects of diabetic model mice that treated with bilayer membrane showed over 80% closure in 8 days. Our findings imply that the double-layer dressing has great potentials to be used in diabetic patients.
Highlights
As the largest human organ, skin is responsible for the physical protection and for sensory detection, thermoregulation, immune surveillance, etc. [1]
There were no intact nucleuses or DNA left on the decellularized bovine amniotic membrane (dBAM)
These results suggested that the sponge-like structure greatly increased thethe hemostatic ability of of wound dressing
Summary
As the largest human organ, skin is responsible for the physical protection and for sensory detection, thermoregulation, immune surveillance, etc. [1]. As the largest human organ, skin is responsible for the physical protection and for sensory detection, thermoregulation, immune surveillance, etc. Skin can be recovered to minimal scar through a complex interaction process. It is quite hard to repair once the wound has become chronic, such as diabetic foot ulcers [2]. Many diabetic patients suffer from the complicate chronic refractory wound [3]. The pathogenesis of diabetic foot ulcers is not well understood, but some work has exhibited that growth factors and immune suppressive cytokine can improve wound healing [4]. The wound dressing is reported to be vital to healing process, mainly because it can protect the wound from external damages as well as provides a suitable microenvironment for tissue regeneration
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