Abstract
Several nutrient permeases have been identified in yeast, which combine a transport and receptor function, and are called transceptors. The Gap1 general amino acid permease and the Mep2 ammonium permease mediate rapid activation by amino acids and by ammonium, respectively, of the protein kinase A (PKA) pathway in nitrogen-starved cells. Their mode of action is not well understood. Both proteins are subject to complex controls governing their intracellular trafficking. Using a split-ubiquitin yeast two-hybrid screen with Gap1 or Mep2 as bait, we identified proteins putatively interacting with Gap1 and/or Mep2. They are involved in glycosylation, the secretory pathway, sphingolipid biosynthesis, cell wall biosynthesis and other processes. For several candidate interactors, determination of transport and signaling capacity, as well as localization of Gap1 or Mep2 in the corresponding deletion strains, confirmed a functional interaction with Gap1 and/or Mep2. Also common interacting proteins were identified. Transport and signaling were differentially affected in specific deletion strains, clearly separating the two functions of the transceptors and confirming that signaling does not require transport. We identified two new proteins, Bsc6 and Yir014w, that affect trafficking or downregulation of Gap1. Deletion of EGD2, YNL024c or SPC2 inactivates Gap1 transport and signaling, while its plasma membrane level appears normal.. Vma4 is required for Mep2 expression, while Gup1 appears to be required for proper distribution of Mep2 over the plasma membrane. Some of the interactions were confirmed by GST pull-down assay, using the C-terminal tail of Gap1 or Mep2 expressed in E.coli. Our results reveal the effectiveness of split-ubiquitin two-hybrid screening for identification of proteins functionally interacting with membrane proteins. They provide several candidate proteins involved in the transport and signaling function or in the complex trafficking control of the Gap1 and Mep2 transceptors.
Highlights
In the yeast Saccharomyces cerevisiae, several nutrient transporters have been identified which combine their transport function with an additional signaling function for rapid activation of the protein kinase A (PKA) pathway in cells starved for the nutrient substrate of the transporter
The transceptors seem to use a non-classical pathway for activation of PKA since cAMP is not required as second messenger in nitrogen and phosphate activation [3,6,7]
Pseudohyphal growth is induced under nitrogen limitation and requires two pathways: the mitogen activated protein kinase (MAPK) pathway and the cAMPPKA pathway
Summary
In the yeast Saccharomyces cerevisiae, several nutrient transporters have been identified which combine their transport function with an additional signaling function for rapid activation of the protein kinase A (PKA) pathway in cells starved for the nutrient substrate of the transporter. Pseudohyphal growth is induced under nitrogen limitation and requires two pathways: the mitogen activated protein kinase (MAPK) pathway and the cAMPPKA pathway (reviewed by [10]). The precise connection between Mep and these two pathways remains unclear, recent results confirm that Mep functions as a transceptor triggering pseudohyphal growth upstream of the MAPK pathway rather than the PKA pathway [11]. The Sch protein kinase is required for nitrogen but not for phosphate activation of the pathway [3]. How it functions precisely in the signaling pathway is not yet clear. Genome-wide expression analysis revealed that Sch most probably acts in a parallel, partially redundant pathway with PKA [12]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
