Abstract
A sensitive and simple method is described for the quantitative determination of free sulfhydryl (-SH) groups on polymer supports. The method includes the reaction of 4,4′-dimethoxytrityloxy- S-(2-thio-5-nitropyridyl)-2-mercaptoethane (DTNPME) with polymer-supported sulfhydryl groups. After removal of excess reagent through washing, a weighed quantity of the polymer support is treated with perchloric acid to release the 4,4′-dimethoxytrityl cation from the polymer support into the solution. The dimethoxytrityl cation (λmax = 498 nm, ϵ 498 = 70,000/ m) is then quantified spectrophotometrically. A comparative study of the reagent DTNPME with 2,2′-dithiobis(5-nitropyridine) is also described.
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