Abstract
A new type of array immunosensor was developed by combining surface plasmon resonance (SPR) and spectral imaging techniques. The system consisted of a monochromator as the wavelength scanning light source, a polarizer, Kretschmann–Raaether attenuated total reflection (ATR) configuration including array sensor chip, and a CCD camera. The images of transmitting light from ATR were recorded versus the wavelength. By averaging gray scales of the pixels in the area of every gold spot from the image series, the complete spectral resonance curve of all sensing spots on the array can be extracted in parallel. The performance of the developed system was evaluated by analyzing interactions of the anti-CD33 monoclonal antibody to its target leukemic cells using 11 cases of human bone marrow specimens. The specimens were also analyzed with flow cytometry method (FCM) for comparison. The initial results measured by the immunosensor array were corresponded with that of FCM, indicating that the developed parallel method might be clinically suitable for immunophenotyping of acute leukemias. The new sensor array system showed the merits of high-throughput, high sensitivity, high specificity, label free and operation convenient. Spots numbers of the array could be increased if suitable technology were adopted for manipulating the micro bio-liquids on the sensor array chip.
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