A specified and accurate RP-HPLC method stimulation and estimation in bulk pharmaceutical dosage forms

Abstract

A specified, accurate and exact RP-HPLC technique is established for the approximation of Hydrochlorothiazide and Losartan Potassium in pharmaceutical dosage form. The novel method was developed by make use of waters HPLC System on a Zodiac C18 column. The Mixture of 0.01% Ortho Phosporic Acid: Acetonitrile 70:30 v/v portable segment is used at a flow rate of 1.5 ml/min. The vaccination capacity selected about 20 µl. The wavelength recognition is approved out at 220 nm. The preservation period of Hydro chlorthiazide and Losartan were originated to be 3.1 min and 7.2 min correspondingly. The established RP- HPLCtechniqueis authenticated as each ICH strategies for the estimation of together the drugs in bulk in addition to in pharmaceutical dosage forms. The established technique is authenticated with regard to Accuracy, Precision, Linearity, Robustness and Ruggedness. Hence, the projected technique is effectively used by simultaneous determination of Hydrochlorothiazide and Losartan Potassium. It is habitually utilized for the medication for Hypertension, congestive heart failure, symptomatic Edema, diabetes Insipidus, renal tubular acidosis. The Thiazide-Type Diuretics (counting Hydrochlorothiazide) would lesquerella powerful over the Thiazide-Like Diuretics (Chlortalidone and Indapamide) for decreasing those danger for heart Attack, Stroke, and heart disappointment in Persons with high bar. HCTZ will be likewise here and there used to prevent Osteopenia Also to medicine of Hypoparathyroidism, hypercalciuria. Thiazides need aid likewise utilized in the medicine about osteoporosis.