A specific sensor system based on in-situ synthesis fluorescent polymers by ARGET ATRP achieving sensitive exosome detection

Abstract

Exosomes, as a kind of tumor biomarker, are often found at elevated levels above normal in the blood of cancer patients, making highly sensitive exosome testing important for monitoring the development and progression of cancer. In this work, an ultra-sensitive and specific fluorescence method for exosome assays was constructed on the basis of atom transfer radical polymerization (ATRP) technique. Firstly, two recognition units, the CD63 aptamer and the EGFR antibody, can specifically bind to exosomes to form a sandwich structure, which endows the proposed sensor with excellent selectivity through two specific recognitions. Secondly, activator regenerated by electron transfer ATRP (ARGET ATRP) polymerization amplification method can introduce a huge amount of the fluorescent monomers into the sensing system, leading to a marked rise in sensitivity. Under optimal experimental conditions, a clear linear relationship between the logarithm of the fluorescence intensity and the concentration of exosomes in the range of 5 × 104 exosomes/mL to 5 × 109 exosomes/mL was obtained. The limit of detection (LOD) was as low as 11,610 exosomes/mL. Furthermore, this sensor exhibited good selectivity and stability, and excellent immunity to interference, providing a new method for the detection of exosomes. The exosome detection strategy used in this system provides a new idea for effective detection of lung cancer at an early stage.