Year-end Sale % OFF 
Abstract
Abstract In this study, we report a facile and cost-effective system for selective measurement of uric acid (UA) using a cascade mechanism composed of Uricase and peroxidase-mimicking trivalent DNAzyme. A three G-quadruplex-hemin complex was engaged as the peroxidase-mimicking DNAzyme. In this cascade catalysis system, UA was oxidized by uricase to produce hydrogen peroxide, which was also acted as a substrate for the oxidation of 3, 3′, 5, 5′ tetramethylbenzidine (TMB) by a peroxidase-mimicking trivalent DNAzyme. TMB oxidation showed a straight relationship with UA concentration. The cascade catalysis system displayed high selectivity toward UA because of the natural selectivity of uricase. This cascade uric acid sensing system revealed the best activity at 30 °C and pH 7.0, which indicated its capacity to uric acid sensing near physiological conditions. The linear regression equation for UA was quantified as A = 0.0072C + 0.0197, with a correlation coefficient of 0.9906. Results suggested that this system could identify as low as 0.66 μM of uric acid with a linear range from 2.5 to 40 μM. Results exhibited that the interfering public components had no noticeable absorbance values. Overall, these results indicated that this facile system would be valuable for uric acid measurement in the medical laboratory.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
