Abstract
An assay procedure specific for the aldotetroses and applicable over a 5- to 200-μg range has been described. It is based on the development of a compound with a 524-mμ absorption maximum when the four-carbon sugars are reacted with phenol in hot concentrated sulfuric acid solution. The reaction conditions are a modification of those previously described for the assay of pentoses and hexoses by the phenol-sulfuric acid technique. The assay conditions described can also be utilized for the assay of these higher sugars simply by altering the level of phenol present and the wavelength employed for the determination. It is also possible to assay aldotetroses in the presence of equivalent levels of hexose or pentose with little interference and to assay the longer chain sugars in the presence of tetrose with only moderate interference. A number of reaction parameters have been studied, and the behavior of several compounds related to the four-carbon sugars under these assay conditions has been described.
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