Abstract
Up to now, most of stimulated-emission-depletion (STED) systems were lens-based bulky systems. Exchanging some spatial light paths with optical fiber components will make the systems more flexible and will benefit various fields. A big problem to achieve this goal is that the STED beam generated by the traditional method of bulky systems cannot be maintained in an optical fiber due to its birefringence. In this article, we will introduce a type of special optical fiber. With the special fiber, a dark hollow beam with doughnut-shaped focal spot and a concentric beam with Gaussian-shaped focal spot can be generated at the same time. Parameters of a sample and a compact STED system based on it are demonstrated.
Highlights
Up to now, most of stimulated-emission-depletion (STED) systems were lens-based bulky systems
Thanks to its non-invasive method to break the diffraction-limited resolution barrier in far-field fluorescence microscopy, much more details can be obtained in biomedical studies in vivo, which promotes the progress of life-science[11,12,13,14,15]
In a typical STED microscopy system, an excitation beam that has a Gaussian-shaped focal spot would be overlapped with a STED beam that has a doughnut-shaped focal spot
Summary
Most of stimulated-emission-depletion (STED) systems were lens-based bulky systems. To align the STED beam with the excitation beam concentrically is a hard work in typical STED systems It seems that if we can use some optical-fiber components to achieve the requirements of STED system, the system will become more compact and flexible. The doughnut-shaped beam generated by the traditional method in bulky STED systems cannot be maintained in a length of optical fiber due to its birefringence, several methods have been reported to achieve doughnut-shaped beam directly from optical fibers[8,17,18,19,20,21,22] These methods still require some specific modulations of incident light (e.g. modulations of phase and polarization state), which inevitably increases the complexity of system. A compact STED fiber system was designed based on this special fiber
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
