Abstract
Evidence is presented for the presence of a new lectin activity in soybean seeds [ Glycine max (L.) Merrill] that has specificity towards the 4-O-methyl- D -glucurono- L -rhamnan exopolysaccharide produced by certain strains of Rhizobium japonicum . Bacterial agglutination and precipitin reactions revealed the lectin activity in phosphate-buffered saline extracts of seeds of all cultivars tested, including the “lectinless” varieties. Reaction of such extracts with carbohydrate haptens demonstrated that the specificity of the binding was towards 4- O -methyl- D -glucuronic acid, D -glucuronic acid and their methyl glycosides.
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