Abstract
Summary A qualitative and quantitative analytical procedure using thin-layer chromatography in combination with fiber optical scanning densitometry is presented for identification of, mainly, flavonol aglycones (quercetin and kaempferol) in extracts from rose leaves. Preliminary cleaning of the extracts was necessary to remove matrix components; SPE was used for this purpose. The compounds were identified by automatic measurement and computer-based comparison of in-situ UV spectra with reference spectra using a cross-correlation function. For amounts between 1 and 10 µg spot–1 there were no differences between in-situ UV spectra acquired for kaempferol but some differences were observed for quercetin.
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