Abstract
A sodium dodecyl sulfate-polyacrylamide gradient gel system is described. It combines a linear gradient in polyacrylamide from 10.2 to 30.2% in the separating gel and the discontinuous ammediol/glycine buffer system suggested by Bury ( A. F. Bury, 1981, J. Chromatogr. 213, 491–500). This urea-free elctrophoretic system provides high resolution and clean separation patterns of proteins and polypeptides with molecular weights from 1500 to 100,000. It is especially suited for studying complex mixtures of proteins and proteolytic fragments, in particular with regard to immunoelectrotransfer blot techniques.
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