Abstract
The outbreak of the new coronavirus (SARS-CoV-2) infection has become a global public health crisis. Antigen detection strips (colloidal gold) can be widely used in novel coronavirus clinical screening and can even be extended to home self-testing, which provides a practical and effective way for people to obtain health status information away from the crowd. In this paper, a colloidal gold detection system without complex devices is proposed, which is based on smartphone usage along with a mobile-phone software embedded with normalization algorithms and a special designed background paper. The basic principle of the device relies on image processing. First, the data of the green channel of the image captured by a smartphone are selected to be processed. Second, the calibration curves are established using standard black and white card, and the calibration values under different detection environments are obtained by calibration curves. Finally, to verify the validity of the proposed method, various standard solutions with different concentrations are tested. Results show that this method can eliminate the influence of different environments on the test results, the test results in different detection environments have good stability and the variation coefficients are less than 5%. It fully proves that the detection system designed in this paper can detect the result of colloidal gold immunochromatographic strip in time, conveniently and accurately in different environments.
Highlights
The antigen for the specific colloidal gold-labeled antibody is fixed on the testing line (T-line), while the antibody for the labeled binding antibody is fixed on the control line (C-line)
It is used to assist the smartp place gold immuno-chromatographic strip (GICS). (2) It provides the image capture area to make sure that the position of the in calibrating the image and is used to achieve four functions: (1) it is us smartphone is relatively stable with the background detection paper
This paper presents a rapid method based on smartphones for colloidal gold im5
Summary
Other traditional detection methods such as chemiluminescent immunoassy, enzyme-linked immunosorbent assay, and electro-chemiluminescence immunoassay require specialized equipment and professional personnel, making them unsuitable for POCT. It is necessary for a cheap, simple, and convenient optical detection method to be developed. It provides the image capture area to make sure that the position o and black card is placed to reduce the interference of external environment on detection, smartphone is relatively stable with that the the background detection paper. It ensures gray value of the detection background isand consistent to guarantee a reasonable exposure time for the smartphone when collecting black card is placed to reduce the interference of external environment on detec images, avoid a very short(4).
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