A small-period long-period fiber grating biosensor based on immobilization of concanavalin A on polydopamine nanospheres for simultaneous detection of D-glucose and temperature

Abstract

The accurate and sensitive monitoring of glucose levels plays a crucial role in diagnosing diabetes in clinical settings. In this work, a novel small-period long-period fiber grating (SP-LPG) biosensor was first developed for D-glucose detection based on the immobilization of concanavalin A (Con A) on polydopamine (PDA) nanospheres. SP-LPG was fabricated by using femtosecond laser direct writing technology and the obtained grating region was only 2.4 mm. The recognition element of D-glucose, Con A, was attached to the surface of PDA nanospheres. Due to the high specific surface area of PDA nanospheres, a large number of binding sites were available for Con A. The as-prepared sensor showed selective and sensitive to D-glucose measurement in the range of 10 nM to 10 mM and a low detection limit of 1.95 nM (S/N = 3) was achieved. Moreover, the small grating period of SP-LPG allows for the observation of Bragg reflection, which can be utilized for temperature sensing. This property eliminates the issue of temperature cross-sensitivity present in refractive index sensing. And the sensor exhibited a thermal sensitivity of 10 pm/°C within the range of 30 ∼ 100 °C. The developed sensor offers a convenient and efficient platform of simultaneously measuring multiple parameters, including target molecules and temperature, for real-time medical monitoring applications.