Abstract
A rapid and reproducible method is described for the simultaneous purification of mononuclear and polymorphonuclear cells, based on the use oof a discontinuous gradient of Percoll. Centrifugation at 600 × g av for 20 min resulted in separation of the mononuclear and polymorphonuclear cells into 2 distinct bands at the interfaces. The upper layer contained the mononuclear cells and the lower band highly purified polymorphonuclear cells. Both populations were purified about 97% without detectable cell alteration.
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