Abstract
BackgroundMarbling score (MS) is the major quantitative trait that affects carcass quality in beef cattle. In this study, we examined the association between genetic polymorphisms of the micromolar calcium-activated neutral protease gene (micro-calpain, CAPN1) and carcass traits in Korean cattle (also known as Hanwoo).ResultsBy direct DNA sequencing in 24 unrelated Korean cattle, we identified 39 sequence variants within exons and their flanking regions in CAPN1. Among them, 12 common polymorphic sites were selected for genotyping in the beef cattle (n = 421). Statistical analysis revealed that a polymorphism in the 3'UTR (c.2151*479C>T) showed significant association with MS (Pcor. = 0.02).ConclusionOur findings suggest that polymorphisms in CAPN1 might be one of the important genetic factors involved in carcass quality in beef cattle, although it could be false positive association.
Highlights
Marbling score (MS) is the major quantitative trait that affects carcass quality in beef cattle
By direct DNA sequencing, 39 polymorphisms were identified within exons and their flanking regions of CAPN1: 6 in coding exons, 10 in 3'UTR, and 23 in introns
12 polymorphisms (c.579G>A [K193K], c.630A>G [T210T], c.760-24T>C, c.843+330A>G, c.1199G>A [R400Q], c.1588G>A [V530I], c.1611+104C>T, c.1869+235C>G, c.2151*479C>T, c.2151*765A>G, c.2151*832G>A, and c.2151*845A>G) were selected for larger-scale genotyping based on location, minor allele frequency exceeding 0.05, Linkage disequilibrium (LD), and haplotype tagging status (Table 2 and Figure 1A)
Summary
Marbling score (MS) is the major quantitative trait that affects carcass quality in beef cattle. We examined the association between genetic polymorphisms of the micromolar calcium-activated neutral protease gene (micro-calpain, CAPN1) and carcass traits in Korean cattle ( known as Hanwoo). Identification of the genes and/or polymorphisms underlying quantitative/qualitative traits, and an understanding of how these genes/polymorphisms interact with the environment or with other genes affecting economic traits might be the keys to successful application of marker-assisted selection in the commercial animal population. As one of these economic traits, marbling is intramuscular fat that gives meat flavor and tenderness.
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