Abstract
AbstractThe sterile insect technique (SIT) has been used to suppress or eradicate fruit flies. It is critical to be able to identify sterile and wild flies so that informed decisions can be made during eradication activities. The current dye marking approach can be flawed on a small number of occasions, and a genetic method is needed to test suspect misidentified samples. As a proof of concept, a single multiplex PCR with nine microsatellite markers was used to study the genetic structure of Queensland fruit flies Bactrocera tryoni (Froggatt) in 11 locations in southern New South Wales. Cluster analysis demonstrated that one cluster was exclusive to the sterile mass‐reared flies. A second distinct cluster was exclusive for one site in a wetter cooler area. The other sites were admixture of two main clusters. These nine microsatellite markers could be used to distinguish laboratory‐reared flies from field flies. The mass‐reared flies would need to be reanalysed after each introduction of wildness.
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