Abstract
The α-amylase mRNAs which accumulate in two different tissues of the mouse, the salivary gland and the liver, are identical except for their 5′ nontranslated sequences: the 5′ terminal 158 nucleotides of the major liver α-amylase mRNA are unrelated to the 5′ terminal 47 nucleotides found in its salivary gland counterpart. DNA that specifies the 5′ terminal one-quarter of these mRNAs has been isolated through genomic cloning and sequenced. The initial 161 nucleotides of the liver α-amylase mRNA are specified by DNA sequences that lie 4.5 kb upstream from those for the common body of the two mRNAs. In contrast, the 5′ terminal 50 nucleotides of the salivary gland α-amylase mRNA are found 7.5 kb from sequences that the two mRNAs share in the genome. These cloned DNA sequences occur once per haploid genome, indicating that both the salivary gland and liver α-amylase mRNAs are transcribed from the same gene ( Amyl A ). Since no rearrangement of these DNA sequences can be detected among mouse sperm, salivary gland or liver preparations, gross rearrangement does not account for the tissue-specific pattern of expression observed for Amyl A . Rather, these data indicate that the salivary gland and liver α-amylase mRNAs are differentially transcribed and/or processed from identical DNA sequences in different tissues.
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