Abstract
Abstract Because of their ability to present antigens, respond to inflammatory signals, and produce critical acute-phase cytokines, renal dendritic cells are of particular interest to those studying various disease states of the kidney. Markers such as CD11c, MHC-II, CX3CR1, and CD11b have historically been used in the murine system to isolate renal dendritic cells from the various parenchymal cells among which they’re embedded. In this study, we have determined that signaling lymphocyte activation molecule family member 9 (SLAMF9) is sufficient to mark the population of CD11b+ MHCIIhi CX3CR1+ CD11c+ F4/80+ cells in the murine kidney under homeostatic conditions. Using SLAMF9, we have devised a single-marker method of renal cDC2 isolation via fluorescence-activated cell sorting (FACS). This method will allow isolation of mouse CD11b+ dendritic cells with minimal surface receptor interference.
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