Abstract
A novel cholic acid-modified pyrene derivative (PyECA) was designed and synthesized. UV–vis absorption spectra and fluorescence emission spectra demonstrate that the fluorescent cholic acid derivative can aggregate strongly in water. Transmission electron microscopy (TEM) measurements show the fluorescent probe forms spherical aggregates with a diameter of ca. 150 nm. Protein sensing studies reveal that the fluorescent supramolecular assembly not only exhibits highly selective ratiometric responses to bovine serum albumin (BSA) and human serum albumin (HSA) among non-metalloproteins, but also illustrates multiple-wavelength cross-reactive quenching responses to metalloproteins. The ratiometric responses are found to be due to the protein binding-induced disassembly of the noncovalent supramolecular assembly, as verified by UV–vis absorption dynamic light scattering, and TEM measurements. The co-factor induced fluorescence quenching of the multiple emission bands provides the sensor the ability to discriminate between metalloproteins. The combination of a fluorophore with multiple emission bands and the ability to form a supramolecular assembly provides a novel and simple strategy for constructing powerful single sensing systems with versatile protein sensing functions.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
