A single-center experience of using immunofluorescence staining of blood smears for the diagnosis of hereditary thrombocytopathies

Abstract

The method of immunofluorescence staining of blood smears is a recently developed approach to the remote diagnosis of various platelet pathologies including MYH9 disorders/MYH9-related disease, biallelic Bernard–Soulier syndrome, Glanzmann thrombasthenia, gray platelet syndrome, and others. We report here the experience of introducing this method at the Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology (Moscow, Russia), which is the main national pediatric hematology hospital that provides diagnosis and therapy to children with blood disorders throughout the country. Our study aimed to transfer this relatively labor-intensive and skill-sensitive method and introduce it into routine laboratory practice, and to perform its validation. The study was approved by the Independent Ethics Committee and the Scientific Council of the Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology.