Abstract
In electroporation, the charging of the cell membrane due to an applied electric field is followed by a localized structural rearrangement of the membrane, which consequently creates pores or aqueous pathways that perforate the membrane. A tremendous increase in ionic and molecular transport through the membrane occurs because of the presence of these aqueous pores. As part of a comprehensive study on the effects of temperature on electroporation of cells, we have designed a micro device capable of integrating single cells into an electronic circuit while controlling the temperature. In the present work, we have studied the effect of temperature on the electroporation of Madin-Darby canine kidney epithelial cells (MDCK) with a micro-electroporation device. It was found that the critical voltage for electropermeabilization was strongly dependent on temperature, increasing by a factor of 2 with decreasing temperature from 37 to 5 °C. Data shows there is a correlation among the viscoelastic properties of the cell membrane and the temperature.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
