Abstract
Single-cell genomics (SCG) methods provide a unique opportunity to analyse whole genome information at the resolution of an individual cell. While SCG has been extensively used to investigate bacterial and archaeal genomes, the technique has been rarely used to access the genetic makeup of uncultivated microbial eukaryotes. In this regard, the use of SCG can provide a wealth of information; not only do the methods allow exploration of the genome, they can also help elucidate the relationship between the cell and intracellular entities extant in nearly all eukaryotes. SCG enables the study of total eukaryotic cellular DNA, which in turn allows us to better understand the evolutionary history and diversity of life, and the physiological interactions that define complex organisms.This article is part of a discussion meeting issue ‘Single cell ecology’.
Highlights
Associations of eukaryotes with archaea and bacteria are central to eukaryote evolution and were the driving force behind the emergence of the eukaryotic cell
An alphaproteobacterial symbiont that once settled within a pre-eukaryotic cell gave rise to mitochondria [1]
As pointed out by Woyke & Schulz [68], we still do not know much about these intimate relationships, and what we do know is heavily skewed towards established laboratory systems, represented by a small number of model hosts that do not reflect the true diversity of eukaryotes or their intracellular associations
Summary
Cite this article: Tyml T, Date SV, Woyke T. 2019 A single-cell genome perspective on studying intracellular associations in unicellular eukaryotes. One contribution of 18 to a discussion meeting issue ‘Single cell ecology’. A single-cell genome perspective on studying intracellular associations in unicellular eukaryotes. While SCG has been extensively used to investigate bacterial and archaeal genomes, the technique has been rarely used to access the genetic makeup of uncultivated microbial eukaryotes. In this regard, the use of SCG can provide a wealth of information; do the methods allow exploration of the genome, they can help elucidate the relationship between the cell and intracellular entities extant in most eukaryotes. This article is part of a discussion meeting issue ‘Single cell ecology’
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