Abstract
The purpose of the present study was to investigate the effect of a single bout of exercise on the capillary growth in an in vitro angiogenesis model and its mechanism. The exercised rats were made to run continuously for 90 min at 20 m/min (0° incline) on a rodent treadmill just before sacrifice. First, we investigated the effect of soleus extracts on the capillary growth in an in vitro angiogenesis model based on the co-culture of rat interstitial cells (like smooth muscle cell) (RSMC) and bovine capillary endothelial cells (BCEC). The extracts of soleus muscle from acutely-exercised rats had higher angiogenic activity, compared with those from control rats. The expression of bFGF mRNA in soleus muscle from acutely-exercised rats was also significantly higher than in that from control rats. Next, we found that norepinephrine (NE) at the concentration of 10 −8 to 10 −5 M enhanced the capillary growth in the angiogenesis model. Furthermore, the addition of 10 −7 M NE markedly enhanced the expression of bFGF mRNA in RSMC, but not in BCEC. The data obtained here suggest that the increased level of NE with physical exercise upregulates the expression of bFGF in satellite cells like RSMC, probably leading to the increased capillarization in skeletal muscle.
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