Abstract
A single amino acid insertion in LCYB2 deflects carotenoid biosynthesis in red carrot.
Highlights
Cyclization of lycopene distinguishes branching point of the carotenoid biosynthesis pathway to complete the metabolic processes in higher plants (DellaPenna and Pogson 2006)
A single amino acid insertion in LCYB2 was found to cause catalytic failure and a flux of lycopene, which may be the molecular basis for the color of red carrot
DcLCYB1, DcLCYB2, and DcLCYE genes from the two-representative orange- (GD2003) and red-colored (D904) inbred lines were tagged with HA and cloned into the same place in the pBluescript SK- vector after the AtLCYB gene was removed (Fig. S5b, Table S1). pAC-LYC was transformed alone as a negative control and co-transformed with lycopene cyclases in E. coli strain TOP10 competent cells
Summary
Cyclization of lycopene distinguishes branching point of the carotenoid biosynthesis pathway to complete the metabolic processes in higher plants (DellaPenna and Pogson 2006). A single amino acid insertion in LCYB2 was found to cause catalytic failure and a flux of lycopene, which may be the molecular basis for the color of red carrot. Carrot harbors two LCYB genes, DcLCYB1 and DcLCYB2, and a functional analysis of DcLCYB1 found that it is crucial for β-carotene biosynthesis as chloroplast-targeted enzymes and the expression level affected plant development and carotenoid abundance (Kössler, et al 2021).
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