Abstract
Yeasts of the genus Kluyveromyces grew very slowly on methylamine as sole nitrogen source. Methylamine oxidase activity in cell-free extracts was very low. Under conditions known to separate methylamine oxidase from benzylamine oxidase in other yeast genera, only a single enzyme was detected in Kluyveromyces lactis. This enzyme could oxidize benzylamine, n-butylamine and (very poorly) methylamine. The enzyme lost no activity on heating at 45°C and had a high affinity and Vmax for benzylamine and 1-aminoalkanes of long-chain length, with a very low affinity and Vmax for methylamine. It is concluded that growth of K. lactis on methylamine involves only benzylamine oxidase, and that a methylamine oxidase of the type found in other yeasts does not occur.
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