Abstract
we present a method for the simultaneous analysis of some common anticonvulsants and their metabolites (phenylethyl malonamide, primidone, ethosuximide, 5-ethyl-5-phenylhydantoin, phenobarbital, tx-methyl-ct-phenylsuccinlmide, mephenytoin, methsuximide, mephobarbital, phenytoin, and carbamazepine) in as little as 25/JI of serum. Serum proteins are precipitated with an acetonitrile solution containing 5-(4methylphanyl)-5-phenylhydantoin, the internal standard. The drugs are eluted from a reversed-phase column with a mobile phase consisting of an acetonitrile/phosphate buffer, at a flow rate of 3.0 ml/min. The eluted drugs are detected by their absorption at 195 nm; their quantities are estimated from their peak heights. Each analysis requires no longer than 30 minutes at the optimum column temperature of 50C. The lower limit of detection for all these drugs is less than 10 ng/sample for drug standards. A sensitivity of 1.0 mglllter of serum is attained routinely for each of the drugs. Analytical recoveries for the eleven drugs varied from 88 to 102%, with good day-to-day precision (CV, between 2.0 and 8.0%). Of more than 35 drugs tested for possible Interference, only ethotoin and pentobarbital interfere with the analysis of phenobarbital and mephobarbital, respectively.
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