Abstract
Fourier ptychography microscopy (FPM) is a recently emerged computational imaging method, which combines the advantages of synthetic aperture and phase retrieval to achieve super-resolution microscopic imaging. FPM can bypass the diffraction limit of the numerical aperture (NA) system and achieve complex images with wide field of view and high resolution (HR) on the basis of the existing microscopic platform, which has low resolution and wide field of view. Conventional FPM platforms are constructed based on basic microscopic platform and a scientific complementary metal–oxide–semiconductor (sCMOS) camera, which has ultrahigh dynamic range. However, sCMOS, or even the microscopic platform, is too expensive to afford for some researchers. Furthermore, the fixed microscopic platform limits the space for function expansion and system modification. In this work, we present a simply equipped FPM platform based on an industrial camera and telecentric objective, which is much cheaper than sCMOS camera and microscopic platform and has accurate optical calibration. A corresponding algorithm was embedded into a conventional FP framework to overcome the low dynamic range of industrial cameras. Simulation and experimental results showed the feasibility and good performance of the designed FPM platform and algorithms.
Highlights
The field of view (FOV) and spatial imaging resolution of a microscopic platform contradict each other
Space–bandwidth product (SBP) [1], the product of FOV and spectral range of the signal entering the optical system, is an important parameter reflecting the throughput of a microscopic system
An effective conventional method to increase SBP is imaging the sample with a high numerical aperture (NA) objective and translating the sample laterally to increase the FOV
Summary
The field of view (FOV) and spatial imaging resolution of a microscopic platform contradict each other. An effective conventional method to increase SBP is imaging the sample with a high numerical aperture (NA) objective and translating the sample laterally to increase the FOV. In the FPM imaging process, the sample is sequentially illuminated by each LED located on different positions of the LED array, and a series of corresponding low-resolution (LR) images are captured simultaneously. Synthetic aperture requires accurate knowledge of the positions and shape of the corresponding spectrum of each low-resolution image. The relative position and azimuth between the LED array, sample, objective, tube lens, and camera need precise calibration. We designed and implemented a equipped FPM platform using an industrial camera and telecentric objective lens. A corresponding FPM algorithm for this system was used to overcome the problem of low dynamic range and high noise of the industrial camera.
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