Abstract
The Hamster Egg Penetration Test (HEPT) is a valuable tool in determining the ability of a patient’s sperm to penetrate an oocyte. One of the challenges of the test is the difficulty in mounting the oocytes to read the test. Oocytes must be flattened to the proper depth to visualize decondensed sperm heads in order to obtain an accurate result without causing the oocytes to lyse. The objective of this study was to evaluate the use of the Makler® chamber and compare it to other methods currently in use for reading the HEPT.
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