Abstract
ABSTRACTAs a collection expands, increasing numbers of test crosses are required to identify new isolates in algal species containing numerous pairs of mating types. A short‐cut is described that utilizes a reduced number of preliminary test crosses. The method was used successfully with the colonial green flagellate, Pandorina morum Bory de St. Vincent, to identify a new pair of milling types (syngen) from Japan and five new isolates from China of a previously known syngen. When tested on the 20 previously described syngens, it revealed one unexpected multi‐clone effect on mating. The method should be valuable for identifying newly collected clones, examining potential, interactions among incompatible mating types, and for screening other highly specific inter‐organism interactions such as host‐parasite infections.
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