Abstract
The gas Chromatographic procedure can be used for the rapid and accurate determination of small amounts of nicotine in biological material. Application of the method proved successful in the determination of the disappearance of nicotine from mouse skin following the application of this alkaloid directly to the skin surface. A plot ofμg of nicotine per mg DNA or protein of mouse skin versus the time in hr following the application of nicotine showed that the biological half-life of nicotine in mouse skin over the first 6 hr is 1.2 hr.
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