Abstract
A simplified method to isolate and cultivate the red alga, Laurencia brongniartii J. Agardh was established to prepare it for indoor tank cultivation. This method was addressed so that relatively inexperienced personnel can start and maintain laboratory cultures of the organism. The method uses the apical tissue of branch tips which are relatively free of epiphytes and requires almost no specialized equipment or facilities. The effect of size and location of apical tissue on the relative growth rates (%RGR) and survival rates (%SR) were examined to determine the size and location of apical tissue that would promote the highest survivability and fastest growth rates. The larger sizes (5.0 mm) showed higher %SR (62.5%) and smaller sizes (1.0 mm) showed higher %RGR (4.43%/day). There was also a significant effect of tissue location, with material closest to the apex showing the highest %RGR (2.16%/day). Different concentrations of nitrate were not significant, however higher concentrations appeared to have a negative impact on %RGR. Temperature and light had a significant effect on %RGR. Optimal growth was observed at 24 and 28°C and at an irradiance of 20μmol photons/m 2 /s.
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